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KMID : 0903519940370030148
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1994 Volume.37 No. 3 p.148 ~ p.153
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A Molecular Study of Rice Black - Streaked Dwarf Virus
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Abstract
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Rice black-streaked dwarf virus (RBSDV), a member of the plant reoviridae fijivirus group, causes a serious damage for rice production in Korea. To characterize the RBSDV genome, virus particles were produced by feeding of planthopper (Laodelphax striatellus F.) earring RBSDV to maize plants for 2 days. In 30∼40 days after feeding, the viral particles were purified from the infected maize roots by using 10∼40% sucrose gradient centrifugation. After treatment of 10% SDS to remove the viral coat proteins, ten viral double-stranded RNAs were resolved in agrose gel electrophoresis. Total dsRNA was then used to synthesize cDNA by reverse transcriptase and a cDNA library was constructed in the λgt11 vector. The phages that contain RBSDV cDNA fragments were selected by hybridizing with the random-primed probe prepared from RBSDV dsRNAs. After subcloning of several cDNA fragments into the pUC19 plasmid mector, one clone (pRV3) was chosen for sequencing. The pRV3 clone was shown to be Located on the RBSDV genome fragment No.3 by RNA gel-blot analysis. Sequence analysis of the clone revealed that the pRV3 contains two partial open reading frames.
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